Pectin decomposing enzyme in genus Erwinia. Purification and properties of pectin lyase.
نویسندگان
چکیده
منابع مشابه
Enzyme assay for identification of pectin and pectin derivatives, based on recombinant pectate lyase.
A simple method was developed for fast identification of pectin, based on a recombinant endopectate lyase cloned from Aspergillus niger. When pectin was demethylated and treated with pectate lyase, beta-elimination occurred, resulting in a double bond between C-4 and C-5 in the galacturonic acid residue of the released nonreducing end. The formation of double bonds produced an increase in light...
متن کاملPurification and Characterization of a Unique Pectin Lyase from Aspergillus giganteus Able to Release Unsaturated Monogalacturonate during Pectin Degradation
A pectin lyase, named PLIII, was purified to homogeneity from the culture filtrate of Aspergillus giganteus grown in submerged culture containing orange peel waste as carbon source. PLIII was able to digest apple pectin and citrus pectins with different degrees of methyl esterification. Interestingly, the PLIII activity was stimulated in the presence of some divalent cations including Pb(2+) an...
متن کاملThe exopolygalacturonate lyase PelW and the oligogalacturonate lyase Ogl, two cytoplasmic enzymes of pectin catabolism in Erwinia chrysanthemi 3937.
Erwinia chrysanthemi 3937 secretes into the external medium several pectinolytic enzymes, among which are eight isoenzymes of the endo-cleaving pectate lyases: PelA, PelB, PelC, PelD, and PelE (family 1); PelI (family 4); PelL (family 3); and PelZ (family 5). In addition, one exo-cleaving pectate lyase, PelX (family 3), has been found in the periplasm of E. chrysanthemi. The E. chrysanthemi 393...
متن کاملGenetic evidence for an activator required for induction of pectin lyase in Erwinia carotovora subsp. carotovora by DNA-damaging agents.
In Erwinia carotovora subsp. carotovora 71, the induction of pectin lyase (Pnl), the bacteriocin carotovoricin (Ctv), and cellular lysis (Lss) requires a RecA function. We obtained mutants wherein a pleiotropic defect, i.e., the lack of induction with mitomycin C, is not restored by the recA+ DNA. From a genomic library of strain 71, a cosmid (pAKC280) that restored induction of Pnl, Ctv, and L...
متن کاملUpdate on Pectin Biosynthesis Biosynthesis of Pectin
Villum Kann Rasmussen Centre Pro-active Plants, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, DK–1871 Frederiksberg C, Copenhagen, Denmark (J.H.); Feedstocks Division, Joint BioEnergy Institute, Emeryville, California 94608 (A.S., H.V.S.); and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Emeryville, California 94608 (...
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ژورنال
عنوان ژورنال: Journal of the agricultural chemical society of Japan
سال: 1988
ISSN: 0002-1407,1883-6844
DOI: 10.1271/nogeikagaku1924.62.1363